Effect of sucrose concentration on in vitro microrhizome production in zingiber zerumbet

Sanasam Thoibi


Zingiber zerumbet popularly known as shampoo ginger is a traditional medicinal plant belonging to Zingiberaceae family and locally known as “singkha” in Manipur (1). The plant is propagated through rhizomes but the rhizome cannot be stored for a longer period of time as it is susceptible to fungal diseases, which affect the quality of the propagules (2). Moreover, the plant is also grown in the wild environment which limit the availability of this plant. The present study will give a simple protocol for in vitro microrhizome production of Zingiber zerumbet which can be used an alternate source for mass propagation of this plant and also studied effect of different concentration of sucrose on the microrhizome formation in Zingiber zerumbet. In vitro microrhizomes of Zingiber zerumbet were produced from in vitro derived microshoots upon transfer to Murashige and Skoog (MS) medium containing various concentration of sucrose (3-9%) with 5 mg/l BAP cultured under 16 hours photoperiod at 25±2oC after twelve weeks of culture. 6% sucrose was found to be most effective concentration of sucrose in the induction of microrhizome in Zingiber zerumbet by giving highest mean (8.33±1.33) number of shoots per explant and also give highest mean (0.435±0.05 g) weight of microrhizome.

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