Antifungal screening, plant growth promoting activities, characterization and analysis of rice seed vigor index of rhizobacteria isolated from the rhizospheric soils of Chakhao Angangbi and Chakhao Wairi

Sajida Sultana

Abstract


Rhizospheric bacteria, having plant growth-promoting ability by colonizing the plant roots, are known as PGPR (Kloepper and Schroth 1978). PGPRs are potentially useful in stimulating plant growth and increasing crop yields (Sayyed et al. 2010). PGPR not only provide essential nutrients for plant growth promotion, but they are also important in biocontrol of pathogens; they also improve soil health in the long term and, can potentially reduce the use of chemical fertilizers and chemical pesticides (Lugtenberg and Kamilova 2009). Black rice is mainly cultivated in limited areas in Manipur as the yield is poor. Therefore, the focus of the present work is isolation of bacteria from rhizospheric soils of Chakhao Angangbi and Chakhao Wairi and screened their PGP potential.
In our present study, 93 rhizobacteria strains were isolated from the rhizospheric soils of Chakhao Angangbi and Chakhao Wairi, screened for their antifungal activity against Rhizoctonia solani (MTCC4633), Fusarium oxysporum (MTCC287), Curvularia oryzae (MTCC2605), Pyricularia oryzae (MTCC1477) and Aspergillus niger (MTCC1344). The bacterial[ cultures showed potent antagonistic activities in dual culture assay. 21 antagonistic isolates were tested for their plant growth promoting (PGP) traits, extra cellular enzyme production, salt and pH tolerance. Two isolates mentionably CR12 and CW11 were most effective which may be useful as biofertilizers, they may enhance the growth of chakhao rice and other rice plants due to the production of ammonia, IAA, HCN, phosphate solubilization, siderophore production and acc deaminase production and also having antifungal activity against phyto pathogenic fungi. Their vigor indices in rice seeds have been performed and the results are significant. Molecular characterization of CR12 and CW11 have also been done.
These rhizobacterial isolates can be promising candidate biocontrol agents for development of rice cultivation. A rhizospheric isolate of Chakhao Wairi, CW11 showed 194μg/ml at pH 3.20 of phosphate solubilisation on quantitative estimation which is in between the phosphate solubilisation index of 30-246 μg/ml in liquid broth (Shumaila Batool et al.. 2019). Again an isolate from rhizospheric soil of Chakhao Angangbi, CR12 produce siderophore of around 73.15% in nutrient broth media that is almost considerable comparing with the previous documented bacteria (Swapan Kumar et al. 2015). Moreover, these cultures showed potential plant growth promoting traits. The pot trial and field trial of CR12 and CW11 are currently underway.


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SAJIDA ET AL

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